Detection of DNA Methylation with Aerolysin Nanopore
نویسندگان
چکیده
منابع مشابه
Nanopore-based assay for detection of methylation in double-stranded DNA fragments.
DNA methylation is an epigenetic modification of DNA in which methyl groups are added at the 5-carbon position of cytosine. Aberrant DNA methylation, which has been associated with carcinogenesis, can be assessed in various biological fluids and potentially can be used as markers for detection of cancer. Analytically sensitive and specific assays for methylation targeting low-abundance and frag...
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DNA methylation is an important epigenetic regulation of gene transcription. Locus-specific DNA methylation can be used as biomarkers in various diseases including cancer. Many methods have been developed for genome-wide methylation analysis, but molecular diagnotics needs simple tools to determine methylation states at individual CpG sites in a gene fragment. In this report, we utilized the na...
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Affiliations: 1 Ontario Institute for Cancer Research, Toronto, Canada 2 Department of Computer Science, University of Toronto, Toronto, Canada 3 Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland Correspondence to: [email protected] [email protected] Abstract Nanopore sequencing instruments measure the change in electric current caused by DNA transi...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2017
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2016.11.1796